BID anticorps (AA 1-195)

N° du produit ABIN7599062

L’anticorps Souris Monoclonal anti-BID a été validé pour WB, IHC, IF, FACS et ICC. Il convient pour détecter BID dans des échantillons de Humain.

Aperçu rapide pour BID anticorps (AA 1-195) (ABIN7599062)

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp BID est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 10F4

Détail du produit anti-BID anticorps

Épitope: AA 1-195

Fonction: Anti-Bid Antibody Picoband® (monoclonal, 10F4)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Bid Antibody Picoband® (monoclonal, 10F4) (ABIN7599062). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequences identity with mouse and rat Bid, respectively.

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur BID

Antigène: BID (BH3 Interacting Domain Death Agonist (BID))

Autre désignation: BID

Sujet:

Synonyms: Apoptic death agonist antibody, Apoptotic death agonist antibody, Apoptotic death agonist BID antibody, BH3 interacting domain death agonist antibody, BH3 interacting domain death agonist p11 antibody, BH3 interacting domain death agonist p13 antibody, BH3 interacting domain death agonist p15 antibody, BH3-interacting domain death agonist p11 antibody, BID antibody, BID isoform ES (1b) antibody, BID isoform L (2) antibody, BID isoform Si6 antibody, BID_HUMAN antibody, Desmocollin type 4 antibody, FP497 antibody, Human BID coding sequence antibody, MGC15319 antibody, MGC42355 antibody, p11 BID antibody, p13 BID antibody, p15 BID antibody, p22 BID antibody

Tissue Specificity: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).

Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Poids moléculaire: 22 kDa

ID gène: 637

UniProt: P55957

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity